Beyond compare tfs3/22/2023 ![]() ![]() KSC Counting Analysis of Cell Subtype-Specific Kinetics Profiles Associated with Different Growth Factor Supplements during Serial Culture a Control value of 30%) and though not statistically significant, a shorter cell cycle time for asymmetric stem cell divisions (8 h vs. These included a statistically significant lower rate of progenitor cell death (6% or less vs. In addition, hPL2 supplemented cells ( Figure 4F) shared three of the differences noted for human sera supplemented cells. Though not statistically significant, hPL2 supplemented cells also had a noticeable lower rate of stem cell symmetric division (48% Figure 4F) compared to Control supplement cells. 67% for Control supplemented cells ( Figure 4E). Their rate of stem cell symmetric division was significantly less, 29% vs. Cells supplemented with hPL1 also showed only one difference. 12), which did not show statistical significance ( Figure 4B). The only difference noted between Control supplemented cells and FBS supplemented cells was a smaller TDN with FBS (7 vs. Blue text, values noticeably different than the respective Control value, but not statistically significant. Red text, values significantly different than the respective Control value. ( A) Control ( B) FBS ( C) HS1 ( D) HS2 ( E) hPL1 ( F) hPL2. (# cells), number of cells in a complete turnover unit. NS, not significantly different than 0.0. %, percent of cells of the labeled type at the start of culture. Bivalent circles, stem cells uniform circles, transiently-dividing committed progenitor cells squares, terminally-arrested cells, amorphous shapes, dead cells. Symmetric stem cell divisions ( left) and asymmetric stem cell divisions ( right) can be distinguished by their cell products. Each diagram depicts the initial cell kinetics factors determined for the evaluated human AdMSC-containing serial cultures supplemented with commercial growth factor sources. Initial cell kinetics factors defined by KSC counting for the tissue stem cell kinetics turnover unit model. KSC Counting Analysis of the Effects of Different Growth Factor Supplements on the Initial Cell Kinetics Factors in the Tissue Stem Cell Kinetics Turnover Unit Model The average DCFs for the Control-, FBS-, and human-sera-supplemented cultures were less than 0.1 ( Figure 2A–D), whereas the average DCF values for the human platelet lysate supplements were somewhat higher ( Figure 2E,F). As shown in Figure 2, a characteristic feature of all the culture supplements is increasing DCF and DCF variability at later serial passages. ![]() These are, respectively, the overall average DCF ( Figure 2A–F, Lines) and the mean coefficient of variation of the DCF means determined at each passage ( Figure 2A–F, mCOV). Two inputs from the DCF data are utilized by the KSC counting software to account for the total number of dead cells produced, which are predominantly terminally-arrested cells present in the cultures, and the variability in their production. The DCF data were determined in parallel at each passage. These results demonstrate the power of the KSC counting method to provide previously inaccessible information for improving future tissue stem cell biomanufacturing. For human sera, both low rates of symmetric division and high rates of stem cell death were responsible. For human platelet lysate, it was attributable to lower rates of self-renewing symmetric stem cell divisions. KSC counting was used to discover the cellular basis for the decreasing stem cell fractions. ![]() In contrast, cultures supplemented with human sera or human platelet lysate showed rapid declines in stem cell fraction. Serial cultures supplemented with the proprietary growth factor product or fetal bovine serum showed a similar high degree of maintenance of stem cell fraction with passage. KSC counting analyses were performed to monitor effects on the fraction and viability of stem cells in serial cultures with their respective supplements. The supplements were a proprietary growth factor product, a source of fetal bovine serum, two sources of pooled human sera, and two sources of human platelet lysate. A recently described kinetic stem cell (KSC) counting method was used to investigate the stem-cell-specific effects of commercial growth factor supplements used for expanding stem cells in adipose-tissue-derived mesenchymal cell preparations.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |